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DNA > DNA MODIFYING ENZYMES

S1 Nuclease Digest of ssDNA

   

Alkaline Phosphatase Digestion of DNA

   

BAP

    CIP

 

S1 Nuclease Digest of ssDNA

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10XS1 buffer:

500mM NaAc pH 4.6

10mM ZnCl2

2.5 M NaCl

For 100ul reaction:

10XS1B 10ul

10XBSA 10ul

DNA 2ug

S1 nuc 10 units

Incubate at 37C for 30 minutes.

Stop reaction by adding 1uL 0.5M EDTA.