> Polymerase Chain Reaction
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For normal PCR, we have found that the following conditions appear to work well.
Set up the following reactions
For DNA stretches of up to 1kbp, then 30" for each step in the cycle is usually enough.
For complete (3-4kbp) plasmids, increase the extension time to 1'30"
C= A x10(6) /E
(11.7x #G's) + (7.3x #C's) + (15.4x #A's) + (8.8x #T's)
Volume to resuspend primer in to get 25 pmol/uL = c/25