Glass Bead Method for Isolating Total Bacteria Proteins
Weigh an empty tube
and add 1.5ml exponential culture O.D. 600 Cells were pelleted
and washed with TDEP buffer. The washed cells are weighed and resuspended in 200 mL of ice-cold TDEP buffer. Add 1 mg prepared
glass beads/mg wet cells to each tube. Each tube was
vortexed for 30 sec. with 30
sec. incubations on ice between vortexing , for a total of 4 times. The cell debris and
DNA was removed by centrifugation for 10 min. in microfuge.
were removed and stored at -20°C.