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Temperature Induction of Large Scale Bacterial Cultures

1. Grow up 500 mL culture in LB + antibiotic at 30C to OD600 0.6-0.8

2. Have equal volume of LB + antibiotic warmed to 54C

3. Induce culture by adding 54C media to cell culture

4. Grow culture at 42C for 2-4 hrs depending on protein expression

5. Separate proteins on SDS-PAGE and analyze