Sensitive between 20 and 1200ug/mL
To measure amounts between 5 and 250ug, incubate at 60º C for 30 minutes, read as above. Assay volume can be adjusted for 120uL proportionally.
High detergent concentrations and high pH do not interfere with this assay.
Tris buffers above 0.1M and glycine buffers, must be adjusted above pH 11. EDTA above 10mM and other strong chelators can interfere.
Assay is a modified Lowry assay, with bicinchonic acid as chelator or cuprous ions.