Sensitive up to 20ug protein, or 25 ug/mL
Can adjust total assay volume to 0.5mL or 100uL in proportion. If measuring about 1mg protein, use 0.1mL sample with 5mL reagent, incubate and read as usual.
Concentrations of detergent higher than 0.1% and high pH levels can interfere significantly with this assay.
Also know as: Bradford assay, Coomassie Brilliant Blue G-250.