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Biological Reagents Protocols


ER / MITO SEPARATION

Separation of Mitochondria and Microsomes

Preparation of Heavy and Light Membrane Fractions From MDCK Cells


ASCITES FLUID

Collecting Ascitic Fluid


ANTIBODIES IN RABBITS

Summary of Rabbit Immunization Procedures

Affinity Purifying Antibody from Rabbit Serum

Purifying Antibodies Made using a GST-fusion Protein


CANINE MICROSOMES

Procedure for Preparing Microsomal Membranes from Dog Pancreas


IgY FROM EGG YOLK

Preparation of IgY from chicken egg yolk


CLL CELL

Ficoll gradient procedure for CLL-cells

P100 membrane prep. of CLL-cells


RIBOSOME PREP

Ribosome(R), S-100 enzymes(S), and peptidyl-tRNA hydrolase(H) preps



Please feel free to try any of our protocols.  They are available in web and Adobe Acrobat format.  All that we ask that you credit us (or the original author) when using them.  This document is in a permanent state of flux - no protocol is ever really perfect and therefore it is never finished!