This section contains just about everything you need to know to assemble plasmids for the expression of cloned genes encoding proteins, fusion proteins and mutant proteins using cell free systems. Some of the protocols are pretty similar to those in standard cloning manuals. However, even for these we have attempted to illustrate protocols in a form that is more convenient.
Please feel free to try any of our protocols. They are available in web and Adobe Acrobat format. All that we ask that you credit us (or the original author) when using them. This document is in a permanent state of flux - no protocol is ever really perfect and therefore it is never finished!