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Overexpression Protocols


OVEREXPRESSION INTRODUCTION


PRIT SYSTEMS

Purification of Fusion Protein on IgG Sepharose 6 FF

Induction of Temperature Sensitive Lambda Repressor


GST FUSIONS

The pGEX System


HIS TAGGED VECTORS

The pRSET System

Purification of His-Tag Proteins

Protocol for BID Protein Purification


PET VECTORS

The pET System


PrA VECTORS

Vectors: Protein A fusion


IMPACT SYSTEM


TOPP CELLS


TEMPERATURE INDUCTION OF LARGE SCALE BACTERIAL CULTURES


INCLUSION BODIES

Purification of Active Eukaryotic Proteins from the Inclusion Bodies in E.Coli

Purification of Proteins from Inclusion bodies

Promoter Variation for Avoiding Inclusion Bodies in Overexpression of Proteins

Cell Strain Variation for Avoiding Inclusion Bodies in Overexpression of Proteins

Sorbitol/Betaine Method for Avoiding Inclusion Bodies in Overexpression of Proteins


EXPRESSION TESTS

Preparing Total Cell Protein

How to Determine Whether Your Protein is Soluble or Insoluble


GLASS BEAD METHOD FOR ISOLATING TOTAL BACTERIAL PROTEINS


HARVEST 500mL

500ml Scale Production of Crude Bacterial Lysate

Large Scale Method: Sonication


INDUCTION PROTOCOL FOR TAC OVERECPRESSION VECTORS


PROTEIN ASSAYS

BCA Protein Assay (Pierce)

BioRad Protein Assay



Please feel free to try any of our protocols.  They are available in web and Adobe Acrobat format.  All that we ask that you credit us (or the original author) when using them.  This document is in a permanent state of flux - no protocol is ever really perfect and therefore it is never finished!