Software analysis of cells is divided into two aspects, Feature Extraction and Object Classification
Feature extraction involves extracting relevant features from the fluorescence micrograph through the use of image segmentation. A variety of features are extracted for each channel. They are broadly classified as "Morphology", "Intensity", "Texture" and "Colocalization". Details of the features can be found here. The script is developed using Acapella(Perkin Elmer) script.
Subtracts the mean intensity of the background from an image. The background is assumed to be the area outside the user-defined object list. By Karsten Kottig,
Evotec. NEW: Also includes a Rolling Ball
like algorithm for background correction.
Schematic illustration of flowchart for CAFE MiCells
We have developed a software capable of classification of the data (which is an output of the feature extractor). The software is called CAFE MiCells . The flow chart is displayed in figure.
The input format is very generic and can be used with any feature extractor. The software is written in MATLAB and involves the use of Statistics, Neural network and bioinformatics toolboxes. The code can be downloaded here. The manual for the software is available here.
Imagej is an image processing and analysis open source software developed by NIH. We were able to extend the features of imagej to analyze FLIM and FRET data . Please download our version of imagej here